Additional file 2: of Genome-wide identification and characterization of abiotic-stress responsive SOD (superoxide dismutase) gene family in Brassica juncea and B. rapa

a Figure depicts the multiple sequence alignment of Cu-ZnSODs from B. juncea, anti-parallel β-strands are shown by over head red arrows, Cu binding residues are shown in yellow asterisk, Zn residues in blue asterisk, Bridging histidine is represented by two asterisk. Also various important structural elements are shown like V-loop (Variable loop with over head yellow line), Electrostatic loop (Green) and Greek key loops 1 and 2 (GK1 and GK2 in purple). The Cu2+ and Zn2+ motif signature sequence is highlighted in black box. b Figure shows the multiple sequence alignment of MnSODs from B. juncea, wherein their metal binding sites is highlighted in black box and the metal binding residues are marked with purple asterisks. Also the α-helices and β-sheets are shown by over head black arrows. c Figure shows the multiple sequence alignment of FeSODs from B. juncea, wherein their metal binding sites is highlighted in black box and the metal binding residues are marked with yellow asterisks. Also the α-helices and β-sheets are shown by over head black arrows. (ZIP 2875 kb)

图a展示了芥菜（Brassica juncea，B. juncea）来源的铜锌超氧化物歧化酶（Cu-ZnSODs）的多序列比对结果：序列上方的红色箭头标注反向平行β折叠链，铜结合残基以黄色星号标记，锌结合残基以蓝色星号标记，桥联组氨酸由双星号标示。此外还标注了多种关键结构元件，包括V环（可变环，以序列上方黄色线条标示）、静电环（绿色标注）以及希腊键环1和2（以紫色标注为GK1与GK2）。Cu²⁺与Zn²⁺基序特征序列以黑色方框高亮显示。
图b展示了芥菜来源的锰超氧化物歧化酶（MnSODs）的多序列比对结果，其金属结合位点以黑色方框高亮，金属结合残基以紫色星号标记。同时以序列上方的黑色箭头标注了α螺旋与β折叠片层。
图c展示了芥菜来源的铁超氧化物歧化酶（FeSODs）的多序列比对结果，其金属结合位点以黑色方框高亮，金属结合残基以黄色星号标记。同时以序列上方的黑色箭头标注了α螺旋与β折叠片层。（压缩包大小2875 kb）