ZNF384 fusion oncoproteins drive lineage aberrancy in acute leukemia [HiChIP]

We report no global changes in H3K27Ac upon expression of ZNF384 fusions but 47 loci with increased H3K27Ac. Therefore we performed HiChIP H3K27Ac to determine if those loci are enhancers. We did not observe any differential looping. 2 replicates of H3K27Ac HiChIP in fusion-expressing or ZNF384 ARF-null pre-B cells.

本研究未观察到ZNF384融合蛋白（ZNF384 fusions）表达后，组蛋白H3赖氨酸27乙酰化（H3K27Ac）出现全局调控变化，但存在47个H3K27Ac水平升高的基因组基因座。因此，我们开展了H3K27Ac的HiChIP实验，以探究这些基因座是否为增强子。本研究未检测到任何差异染色质环。本实验针对表达ZNF384融合蛋白的前B细胞与ARF缺陷型ZNF384前B细胞两类样本，均设置了2次生物学重复的H3K27Ac HiChIP实验。