rCRUX Generated Ford Fish 16S Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: Ford Fish 16S<br> Gene: 16S<br> Length of Target: 330<br> get_seeds_local() minimum length: 279<br> get_seeds_local() maximum length: 477<br> blast_seeds() minimum length: 231<br> blast_seeds() maximum length: 429<br> max_to_blast: 1000<br> Forward Sequence (5'-3'): GCAATCACTTGTCTTTTAAATGAAGACC, GTAATCACTTGTCTTTTAAATGAAGACC<br> Reverse Sequence (5'-3'): GGATTGCGCTGTTATCCCTA<br> Reference: Ford MJ, Hempelmann J, Hanson MB, Ayres KL, Baird RW, et al. (2016) Estimation of a Killer Whale (Orcinus orca) Population’s Diet Using Sequencing Analysis of DNA from Feces. PLOS ONE 11(1): e0144956. https://doi.org/10.1371/journal.pone.0144956 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.

本参考数据库由rCRUX生成，所用数据为2022年12月下载的NCBI核苷酸BLAST（NCBI nt BLAST）数据库。
引物名称：Ford Fish 16S
基因：16S
靶标片段长度：330
get_seeds_local()最小长度：279
get_seeds_local()最大长度：477
blast_seeds()最小长度：231
blast_seeds()最大长度：429
最大BLAST比对序列数：1000
正向引物序列（5'-3'）：GCAATCACTTGTCTTTTAAATGAAGACC、GTAATCACTTGTCTTTTAAATGAAGACC
反向引物序列（5'-3'）：GGATTGCGCTGTTATCCCTA
参考文献：Ford MJ、Hempelmann J、Hanson MB、Ayres KL、Baird RW等（2016）利用粪便DNA测序分析估算虎鲸（Orcinus orca）种群食性。PLOS ONE 11(1): e0144956。https://doi.org/10.1371/journal.pone.0144956
本研究为<em>get_blast_seeds()</em>选用rCRUX默认参数：覆盖度70%、序列一致性70%、E值3e+7，最大BLAST比对数为'100000000'；为<em>blast_seeds()</em>选用的默认参数为：覆盖度70%、序列一致性70%、E值3e+7、属水平分类阶元，最大BLAST比对数为'10000000'。