RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

The aim of this experiment was to observe the transcriptional profile of individual populations of cells in mouse islets throughout development. MARIS was used to isolate Ins+ islet cells at E18.5 and P13, and further separate Ucn3- and Ucn3+ populations at the transition point P4 and P5.本实验旨在观测小鼠胰岛内各细胞亚群在整个发育过程中的转录谱。实验采用MARIS技术，于胚胎第18.5天（E18.5）与出生后第13天（P13）分离胰岛素阳性（Insulin+）胰岛细胞，并在出生后第4天（P4）至第5天（P5）的发育过渡节点中，进一步分离得到尿皮质素3（Urocortin 3）阴性（Ucn3-）与尿皮质素3（Urocortin 3）阳性（Ucn3+）细胞群。