COI metabarcoding sequences of seaside sparrow (Ammospiza maritima) fecal samples and gizzard contents collected from oiled, unoiled, and reference sites in in Plaquemines Parish, Louisiana, 2016-06-08 to 2017-06-29

Seaside sparrows were captured in Plaquemines Parish, Louisiana, on oiled, unoiled, and reference sites in June 2016 and 2017. Fecal samples were opportunistically collected from all captured birds and gizzard samples were collected from every dissected bird. Prey DNA was extracted from these samples and sequenced on an Illumina MiSeq to generate amplicon sequences of a portion of the COI gene. This metabarcoding (or amplicon) data will allow us to identify prey species in sparrow diet on oiled, unoiled, and control sites, as well as identify any variation in described diet attributed to the sample type used (gizzard or fecal). Sample collection data is reported and COI metabarcoding data is located at NCBI, BioProject PRJNA629391.

2016年与2017年6月，研究人员于美国路易斯安那州普拉克明兹堂区（Plaquemines Parish）的受油污染、未受油污染及对照样点中捕获海滨麻雀（Seaside sparrow）。研究人员采用偶遇采样策略采集所有捕获个体的粪便样本，并对每只剖检个体采集砂囊样本。随后从上述样本中提取猎物DNA，依托Illumina MiSeq测序平台完成测序，以获取COI基因（细胞色素c氧化酶亚基I，Cytochrome c oxidase subunit I）部分区段的扩增子（amplicon）序列。本宏条形码（metabarcoding，或扩增子）测序数据可用于鉴定受油污染、未受油污染及对照样点中海滨麻雀的猎物物种，同时可分析因样本类型（砂囊或粪便）不同导致的已记录食性差异。本数据集附带样本采集相关数据，COI宏条形码测序数据已上传至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的BioProject数据库，登录号为PRJNA629391。