NK cell analysis in the griddient

HCT-116 cells (blue) were seeded at 30 million cells/mL in a collagen solution. A biopsy of the gel was isolated, and the cavity was filled with a hydrogel containing NK-92 cells (green). IP was added into the media to visualize dead cells. Images was obtained in a fluorescence microscope at day 0 and 15 after the addition of NK-92 cells. Quantification of the NK cell number and area covered by NK cell at day 0 and 15. Data was normalized by area covered at day 0 and plot as as mean ±SEM, n=3 . .

将蓝色标记的HCT-116细胞以3×10^7个细胞/毫升的密度接种于胶原溶液中。分离得到该凝胶的活检样本，并向其空腔中填充搭载有绿色标记NK-92细胞的水凝胶。向培养基中加入碘化丙啶（IP）以可视化死细胞。于添加NK-92细胞后的第0天和第15天，利用荧光显微镜采集实验图像。分别在第0天与第15天，对NK细胞数量及NK细胞所覆盖的区域面积进行定量分析。以第0天的覆盖面积作为基准对数据进行归一化处理，随后以平均值±标准误（mean ± SEM）的形式绘制图表，实验重复次数n=3。