RMSD and fluorimeter data for "Structural transitions in kinesin minus-end directed microtubule motility"

This entry is for our manuscript, "Structural transitions in kinesin minus-end directed microtubule motility" by Satoki Shibata*, Matthew Y. Wang*, Tsuyoshi Imasaki*, Hideki Shigematsu, Yuanyuan Wei, Chacko Jobichen, Hajime Hagio, J. Sivaraman, Sharyn A. Endow & Ryo Nitta.

*Equal contribution

Corresponding Authors: Tsuyoshi Imasaki*, Sharyn A. Endow, Ryo Nitta

The deposited datasets are 1) beta-strand residue all atom RMSD between kinesin-14 NcdY485K ADP and ADP + free Pi nucleotide states and 2) fluorimeter assays of changes in intrinsic NcdY485K fluorescence upon addition of free Pi . The data are presented in Fig. 7e, and Fig. 8b,c and Sup Fig 2, respectively. Files are named for the figures in which the data are shown. A computer script for the RMSD analysis is included. Methods for the fluorimeter assays and data analysis are given.

本条目对应我们的学术论文《驱动蛋白负端定向微管运动的结构转变》（Structural transitions in kinesin minus-end directed microtubule motility），作者为Satoki Shibata*、Matthew Y. Wang*、Tsuyoshi Imasaki*、Hideki Shigematsu、Yuanyuan Wei、Chacko Jobichen、Hajime Hagio、J. Sivaraman、Sharyn A. Endow与Ryo Nitta。
* 共同第一作者
通讯作者：Tsuyoshi Imasaki*、Sharyn A. Endow、Ryo Nitta

本次提交的数据集包含两项内容：1）驱动蛋白-14 NcdY485K在腺苷二磷酸（ADP）结合态以及ADP+游离无机磷酸（Pi）核苷酸结合态下，β折叠（beta-strand）残基的全原子均方根偏差（all atom RMSD）；2）添加游离Pi后NcdY485K内源荧光变化的荧光分光光度测定实验数据。
上述数据分别对应图7e、图8b、8c以及补充图2。数据文件以其对应展示的图表编号命名。本次提交包含用于均方根偏差分析的计算机脚本，同时提供了荧光分光光度测定实验及数据分析的相关方法。