Proteomic analysis of senescent and non-senescent CHME-5 microglia.

In this experiment, senescent CHME-5 microglial cells were generated and their proteomes were compared to non-senescent CHME-5 microglial cells. Compared to non-senescent cells, a detailed proteomic analysis using SWATH-MS identified 201 proteins that were significantly downregulated and 127 that were significantly upregulated in senescent cells.

本实验中构建了衰老型CHME-5小胶质细胞，并将其蛋白质组与非衰老型CHME-5小胶质细胞的蛋白质组进行对比。相较于非衰老型细胞，采用SWATH-MS技术完成的详细蛋白质组学分析鉴定出，衰老型细胞内共有201种蛋白质显著下调、127种蛋白质显著上调。