Additional file 13 of CTCF is selectively required for maintaining chromatin accessibility and gene expression in human erythropoiesis
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Additional file 13: Table S12. Oligonucleotides, DNA primers, and antibodies used in this study. Oligonucleotides were used for sgRNA vector construction; DNA primers were used to analyze Cas9-mediated indels by Sanger sequencing; antibodies were used for Western blotting, immunoprecipitation, or ChIP-seq analysis.
补充材料13:表S12。本研究中使用的寡核苷酸、DNA引物及抗体。其中,寡核苷酸用于sgRNA(single-guide RNA)载体构建;DNA引物用于通过桑格测序(Sanger sequencing)分析Cas9介导的插入缺失(indels);抗体用于蛋白质印迹法(Western blotting)、免疫沉淀(immunoprecipitation)或染色质免疫沉淀测序(ChIP-seq)分析。
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figshare
创建时间:
2025-03-01



