Identification of genes regulated by Long noncoding RNA H19 in hepatic cells. Homo sapiens

We used high throughput sequencing to compare the differential gene expression of HepG2 cells with and without H19 knockdown. We found critical genes involved in glucose production changed significantly after H19 konckdown compared to control. Overall design: HepG2 cells were transfected with either control siRNA or siH19. 48h after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare trancript profiles between siCon and siH19 cells.

本研究采用高通量测序（High-throughput sequencing）技术，对比分析了存在H19基因敲低（H19 knockdown）与未敲低的HepG2细胞的差异基因表达情况。研究发现，与对照组相比，经H19敲低处理后，参与葡萄糖生成的关键基因的表达发生了显著改变。整体实验设计如下：将HepG2细胞分别转染对照小干扰RNA（control siRNA）与靶向H19的小干扰RNA（siH19）；转染48小时后，提取总RNA进行文库构建与RNA测序（RNA-seq）分析，以对比siCon组与siH19组细胞的转录本表达谱。