Ancient fish lineages place innate immune receptors into the broader context of vertebrate molecular evolution

Polypteriformes are the earliest diverging ray-finned fishes. The early divergence of this lineage offers critical context concerning the early evolution of innate immune receptors and other gene families for half of all living vertebrates. To augment genomic resources for this clade, kidney, intestine, and spleens from two Polypterus senegalus were dissected and RNA from each tissue type was extracted (Trizol, Life Technologies). Equal amounts of RNA were pooled together prior to NGS library prep.

多鳍鱼目（Polypteriformes）是现存最早分化的辐鳍鱼类。该演化支的早期分化事件，为解析占现存脊椎动物半数的类群的先天免疫受体及其他基因家族的早期演化提供了关键研究背景。为丰富该演化支的基因组资源，研究人员从两条塞内加尔多鳍鱼（Polypterus senegalus）体内解剖获取肾脏、肠道与脾脏组织，并从每种组织中提取核糖核酸（RNA）（采用TRIzol试剂，Life Technologies公司）。在开展下一代测序（Next Generation Sequencing, NGS）文库构建前，将等量的各组织RNA进行混合。