Data_Sheet_1_Anti-phytopathogenic Bacterial Metabolites From the Seaweed-Derived Fungus Aspergillus sp. D40.docx

In order to search for new lead compounds with anti-phytopathogenic bacterial activity, three pairs of new furanone derivatives, sclerotiorumins D–F (1–3), and eight known compounds (4–11) were isolated from the seaweed-derived fungus, Aspergillus sp. D40, fermented with potato dextrose seawater (PDW) medium. Their structures were determined using comprehensive spectroscopic analyzes including HRESIMS, 1D and 2D NMR data. Compounds 1–4 and 9 existed as inseparable mixtures of a pair of epimers. Penicillic acid (7) exhibited clear antibacterial activity against Ralstonia solanacearum and several other plant pathogenic bacteria with IC50 values ranging from 11.6 to 58.2 μg/mL.

为筛选具有抗植物病原细菌活性的新型先导化合物，本研究从经马铃薯葡萄糖海水（PDW）培养基发酵的海藻来源真菌曲霉属（Aspergillus sp.）D40菌株中，分离得到三对新型呋喃酮衍生物sclerotiorumins D–F（1–3）以及8个已知化合物（4–11）。通过高分辨电喷雾电离质谱（HRESIMS）、一维及二维核磁共振波谱等综合波谱分析手段，确定了所有化合物的化学结构。化合物1–4与9以一对不可分离的差向异构体混合物形式存在。青霉酸（Penicillic acid，7）对青枯劳尔氏菌（Ralstonia solanacearum）及其他多种植物病原细菌表现出显著的抗菌活性，其半数抑制浓度（IC50）范围为11.6至58.2 μg/mL。