Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells

Multiple myeloma RPMI8226 cells adapted to growth in melphalan display a shift towards Warburg metabolism and modulated oxidative stress signaling Inhibitors targeting specific enzymes in these pathways are selectively toxic to the melphalan-resistant cells. The gene expression profiles were measured on 6 batches each of control and melphalan-treated RPMI8226 and RPMI8226-LR5 cells using Illumina Human HT-12 v3 Expression BeadChip (Illumina, San Diego, CA), which enables genome-wide expression analysis (more than 47 000 transcripts) of 24 samples in parallel on a single microarray.

适应美法仑（melphalan）培养的多发性骨髓瘤（Multiple myeloma）RPMI8226细胞，呈现出向瓦博格代谢（Warburg metabolism）的表型转变，并伴随氧化应激信号通路的调控异常。靶向此类通路中特定酶的抑制剂，对美法仑耐药的该细胞株具有选择性细胞毒性。本数据集采用Illumina Human HT-12 v3表达微珠芯片（Illumina Human HT-12 v3 Expression BeadChip，Illumina, 美国加利福尼亚州圣地亚哥），对6个实验批次的RPMI8226对照组、美法仑处理组，以及RPMI8226-LR5细胞的对照组、美法仑处理组进行了基因表达谱检测；该芯片可在单张微阵列上并行完成24个样本的全基因组表达分析，覆盖超过47000个转录本。