Hinesetal_BMCGenomics_fulldatainfo

This file includes accessory information on each transcript contig used from the associated microarray study, including annotation results from various analyses, mean values across probes for that gene across the tested conditions, and significance across the variety of statistical analyses performed. By column this dataset includes: | Transcript ID – The identifications assigned to each transcript on the array. These IDs are referenced in the text and sequences for each contig are available at <http://insectacentral.org>. | Blast2Go Annotation – Annotation term assigned during the Blast2Go analysis of these transcripts. | Flybase Gene ID and Flybase Annotation Abbr. – The gene ID the contig blasted to in Flybase and the name assigned to that gene. | Manual Anno Flybase Gene ID and Manual Anno Flybase Anno Abbr. – Flybase gene identifications and terms for genes that did not hit to Flybase with the contig sequence but were whose identity were determined manually using various parameters outlined in the manuscript. | Significant for Hindwing, Significant for Forewing – If an ID is listed, that gene was significant in either the hindwing or forewing analyses. | Lsmean columns – These provide the mean expression across probes across each array condition, including in the title Race, Stage, and (for forewings) Wing Section. | -log10(p-value) columns– These provide the p-values from ANOVAs for the two listed pairwise comparisons of those Lsmeans | Significant Index columns – Indicate whether p-values of various pairwise comparisons were significant (1) or not (0). | Columns FR – GT- IDs are listed where significant differences were found with various analyses, described in the manuscript. | Columns GU – HM – These refer to the genes that are differentially expressed in an ANOVA along the proximal-distal forewing axis and the significance columns refer to whether there was significance for each of the wing section comparisons. F1 is the basal section, F2 is the medial section, and F3 is the apical section.

本文件包含了来自相关微阵列研究（microarray study）中所用的每一条转录重叠群（transcript contig）的辅助信息，涵盖各类分析的注释结果、待测条件下该基因对应探针的平均表达值，以及各类统计分析得到的显著性结果。本数据集各列的含义如下：
- 转录本ID（Transcript ID）：为芯片上每条转录本分配的唯一标识。文中均引用该ID，各重叠群的序列可在<http://insectacentral.org>获取。
- Blast2Go注释（Blast2Go Annotation）：对这些转录本进行Blast2Go分析时赋予的注释术语。
- Flybase基因ID及Flybase注释缩写（Flybase Gene ID and Flybase Annotation Abbr.）：该重叠群在Flybase数据库中比对到的基因ID，以及该基因的官方命名缩写。
- 手动注释Flybase基因ID及手动注释Flybase注释缩写（Manual Anno Flybase Gene ID and Manual Anno Flybase Anno Abbr.）：针对那些无法通过重叠群序列比对到Flybase，但通过手稿中提及的各类参数手动鉴定得到其身份的基因，对应的Flybase基因标识与注释术语。
- 后翅显著性、前翅显著性（Significant for Hindwing, Significant for Forewing）：若列中存在标识，则说明该基因在后翅或前翅分析中具有统计学显著性。
- 最小二乘均值列（Lsmean columns）：提供各芯片实验条件下对应探针的平均表达量，列标题包含品系（Race）、发育阶段（Stage），以及前翅样本的翅区（Wing Section）信息。
- -log10(p值)列（-log10(p-value) columns）：提供针对上述最小二乘均值的两组指定成对比较的方差分析（ANOVA）得到的p值转换结果。
- 显著性索引列（Significant Index columns）：标记各类成对比较的p值是否显著，显著记为1，不显著记为0。
- FR列：列中列出手稿中描述的经各类分析证实存在表达差异显著的GT-ID。
- GU至HM列：对应经方差分析（ANOVA）鉴定出沿前翅近-远端轴存在差异表达的基因，其右侧的显著性列用于标记各翅区比较是否具有统计学意义。其中F1代表翅基区，F2代表翅中区，F3代表翅尖区。