Assessment of anti-neutrophil cytoplasmic antibodies (ANCA) and ANCA against proteinase 3 (PR3) and myeloperoxidase (MPO) by classical and automated multiplex analysis in one reaction environment.

ANCA were determined by enzyme-linked immunosorbent assay (ELISA), classical indirect immunofluorescence (IIF) on ethanol-fixed neutrophils and multiplex CytoBead assay in patients and controls: 118 patients with ANCA-associated vasculitis, 300 with autoimmune and gastrointestinal diseases, 49 with infectious disorders, and 125 blood donors (BD).* pAIH, autoimmune hepatitis; BD, blood donors; CD, Crohn's disease; c/p ANCA, cytoplasmic/perinuclear ANCA; GPA, granulomatosis with polyangiitis; ID, infectious diseases; MIA, microbead immunoassay; MPA, microscopic polyangiitis; N, number; PSC, primary sclerosing cholangitis; RA, rheumatoid arthritis; UC, ulcerative colitis.Assessment of anti-neutrophil cytoplasmic antibodies (ANCA) and ANCA against proteinase 3 (PR3) and myeloperoxidase (MPO) by classical and automated multiplex analysis in one reaction environment.

本研究通过酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）、乙醇固定中性粒细胞经典间接免疫荧光试验（classical indirect immunofluorescence, IIF）以及多重CytoBead检测法，对受试患者与对照人群的抗中性粒细胞胞浆抗体（anti-neutrophil cytoplasmic antibodies, ANCA）进行检测，共纳入118例ANCA相关血管炎患者、300例自身免疫及胃肠道疾病患者、49例感染性疾病患者，以及125名献血者（blood donors, BD）。* 注：pAIH即自身免疫性肝炎（autoimmune hepatitis）；BD即献血者（blood donors）；CD即克罗恩病（Crohn's disease）；c/p ANCA即胞浆/核周型ANCA（cytoplasmic/perinuclear ANCA）；GPA即肉芽肿性多血管炎（granulomatosis with polyangiitis）；ID即感染性疾病（infectious diseases）；MIA即微球免疫测定法（microbead immunoassay）；MPA即显微镜下多血管炎（microscopic polyangiitis）；N即例数（number）；PSC即原发性硬化性胆管炎（primary sclerosing cholangitis）；RA即类风湿关节炎（rheumatoid arthritis）；UC即溃疡性结肠炎（ulcerative colitis）。本研究在单一反应体系中，采用经典分析与自动化多重分析技术，对抗中性粒细胞胞浆抗体（ANCA）以及针对蛋白酶3（proteinase 3, PR3）和髓过氧化物酶（myeloperoxidase, MPO）的ANCA进行检测。