shoots and roots treated with methotrexate and 5-formyl-thf-Transcriptomic and metabolic changes in Arabidopsis thaliana supplemented with 5-CHO-THF and its relationship with the accumulation and transformation to THF active derivatives. Arabidopsis thaliana

au11-02_cho-thf - shoots and roots treated with methotrexate and 5-formyl-thf - Folates and 1C metabolism - The objective of this study is to investigate the changes on the plant expression profiles due to 5-CHO-THF assimilation and transformation. Additionally, plants supplemented with methotrexate (MTX) (an inhibitor of folate biosynthesis) will complement the study. Results from this work will serve to better understand the effects of folate accumulation in plants. Overall design: 12 dye-swap - treated vs untreated comparison

样本编号au11-02_cho-thf——经甲氨蝶呤（methotrexate, MTX）与5-甲酰基四氢叶酸（5-formyl-THF）处理的植株地上部与根系——研究领域：叶酸类物质与一碳代谢（Folates and 1C metabolism）。本研究旨在探究5-甲酰基四氢叶酸（5-CHO-THF）的同化与转化过程对植物基因表达谱的影响。此外，本研究补充设置了施加叶酸生物合成抑制剂甲氨蝶呤（MTX）的实验组以辅助研究。本研究结果将助力更深入地解析叶酸类物质在植物体内的积累效应。整体实验设计：开展12组染料置换（dye-swap）实验，进行处理组与未处理组的对照比较。