Two different 4-week energy-restricted diets.

The main aim of this experiment was to investigate gene expression on human adipose tissue after two different 4-week energy-restricted diets. Our questions consisted in understanding how gene expression was linked to clinical parameters of obese patients and whether the two diets were discriminated this data. The subjects were randomly allocated, in a cross-over design, to two periods of 4 weeks of an energy restricted isocaloric diet of 1200 kcal as either a conventional diet (LC-CONV) or a special energy restricted diet compensated by proteins (LC-P-LGI). The two nutritional periods were separated by a wash-out interval of 8 weeks. The samples who contain a K in their name correspond to the LC-P-LGI diet while those who contain a C correspond to LC-CONV diet. Total RNA obtained from isolated human adipose tissue of 12 subjects at 6 different time points (0, 5, 30, 90, 95 and 120 days).

本实验的核心目标为探究两种不同的4周能量限制饮食（energy-restricted diets）干预后，人类脂肪组织的基因表达情况。本研究旨在明确基因表达与肥胖患者临床参数的关联，以及两种饮食能否通过该数据实现区分。受试者采用交叉试验设计（cross-over design），被随机分配至两个为期4周的能量限制等热量饮食（isocaloric diet）干预阶段，每日热量摄入为1200 kcal，分别为常规饮食（LC-CONV）与蛋白质补偿型特殊能量限制饮食（LC-P-LGI）。两个营养干预阶段之间设置8周的洗脱期（wash-out interval）。样本名称中含K的对应LC-P-LGI饮食组，含C的则对应LC-CONV饮食组。研究从12名受试者的分离人类脂肪组织中提取总RNA（total RNA），共采集了6个不同时间点（0、5、30、90、95和120天）的样本。