EdU Optimization - Concentration Tests - 10-35 um EdU

Flow cytometry data on mouse fecal bacteria incubated with varying concentrations of 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, to capture the actively replicating bacteria.

The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria.

本数据集包含小鼠粪便细菌的流式细胞术检测数据：实验中使用不同浓度的胸腺嘧啶类似物5-乙炔基-2'-脱氧尿苷（5-ethnyl-2'-deoxyuridine, EdU）对细菌进行孵育，以捕获活跃增殖的细菌。

细菌细胞与Alexa Fluor 647叠氮化物（APC通道）进行点击化学反应，使新增殖的细菌发出荧光；同时使用Sybr Green I核酸染料（FITC通道）对所有细菌进行染色标记。