Expression data from rhesus macaque jejunum

The mucosa that lines the gastrointestinal (GI) tracts is an important portal of entry for pathogens and provides the frontline of immune defense against HIV infection. Epithelial barrier dysfunction during HIV infection has largely been attributed to the rapid and severe depletion of CD4 T cells in the gastrointestinal (GI) tract. In this study, the poential role of small non-coding microRNA (miRNA) to contribute to epithelial dysfunction was investigated in the non-human primate SIV model and microarrays were utilized to determine changes in mucosal gene expression (non-miRNA) that could be correlated to miRNA modulatiolns. Microarrays were used to characterize changes in gene expression in the jejunal mucosa that occur during chronic SIV infection Jejunum tissues from healthy uninfected macaques and macaques with chronic stage SIV infection were used for RNA extraction and hybridization on Affymetrix microarrays.

胃肠道（gastrointestinal, GI）黏膜作为病原体侵入的重要门户，同时是抵御HIV感染的免疫防御前线。HIV感染期间出现的上皮屏障功能障碍，在很大程度上被归因于胃肠道内CD4 T细胞的快速重度耗竭。本研究以非人类灵长类动物猴免疫缺陷病毒（Simian Immunodeficiency Virus, SIV）感染模型为对象，探讨了小型非编码微小RNA（microRNA, miRNA）介导上皮功能障碍的潜在作用；同时利用基因芯片技术，明确可与miRNA调控相关的黏膜非miRNA类基因的表达变化。本研究采用基因芯片技术表征慢性SIV感染期间空肠黏膜的基因表达变化，实验所用组织取自健康未感染猕猴与慢性期SIV感染猕猴的空肠组织，经RNA提取后在Affymetrix基因芯片上完成杂交实验。