Single cell RNA-seq of single cell suspension from the HCC region of WT and FABP5 Hepatocyte KO mice fed CD-HFD for 15 month

After feeding CD-HFD diet for 15 month, tumors from WT and FABP5 hepatocyte KO mice were collected and digested with collagenase to generate single cell suspension before proceeding with 10X Single Cell RNA-Sequencing. The tumor region of WT and FABP5 Alb-CRE-ER mice were dissected after 15 month of CD-HFD. Single cell suspension was prepared and cells were submitted for sc-RNAseq

将喂食15个月高胆固醇高脂饮食（Cholesterol-rich High-Fat Diet, CD-HFD）的野生型（Wild Type, WT）及肝细胞特异性脂肪酸结合蛋白5（Fatty Acid Binding Protein 5, FABP5）敲除小鼠的肿瘤组织收集，经胶原酶（collagenase）消化制备单细胞悬液（single cell suspension）后，进行10×单细胞RNA测序（10X Single Cell RNA-Sequencing）。在喂食15个月CD-HFD后，对野生型及FABP5 Alb-CRE-ER小鼠的肿瘤区域进行解剖，制备单细胞悬液并提交开展单细胞RNA测序（sc-RNAseq）。