SARS-CoV-2 proteins BioID in HEK293

SARS-CoV-2 proteins orchestrate complex mechanisms that underlie SARS-CoV-2 infection and COVID-19 causing pathogenic processes. Here we have exhaustively defined human proteins in spatial proximity (proximal interaction network) of 27/28 SARS-CoV-2 proteins in human cells by proximity-dependent biotinylation (BioID)-based approach. We report 10,185 high-confidence virus-host PPI involving 2,592 unique cellular targets.

严重急性呼吸综合征冠状病毒2（SARS-CoV-2）蛋白可调控介导该病毒感染与新型冠状病毒肺炎（COVID-19）致病进程的复杂分子机制。本研究通过基于邻近依赖生物素标记（BioID）的实验方法，在人类细胞中全面鉴定了与27/28个SARS-CoV-2蛋白存在空间邻近关系（即邻近互作网络，proximal interaction network）的人类宿主蛋白。本研究共报道了10185个高置信度的病毒-宿主蛋白-蛋白相互作用（Protein-Protein Interaction, PPI），涉及2592个独特的宿主靶标蛋白。