Additional file 3 of Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)

Additional file 3: Figure S1. Gene ontology classification of the assembled unigenes. The Y-axis indicates the number of unigenes and X-axis indicates the GO categories. Figure S2 Functional classification of KEGG pathways of the assembled unigenes. The KEGG pathways were classified into six functional categories: A- Metabolism; B- Genetic Information Processing; C- Environmental Information Processing; D- Cellular Processes; E- Organismal Systems; F- Human Diseases. The Y-axis represents the KEGG metabolic pathways. The X-axis represents number of unigenes annotated in that particular pathway. Figure S3 Hierarchical tree graph of over-represented GO terms in the biological process category of down-regulated DEGS. Boxes in the graph represent GO terms labeled according to their GO ID, term definition, and statistical information. Significant terms (adjusted P ≤ 0.05) are in color (red, orange, or yellow), while non-significant terms are shown as white boxes. In the diagram, the degree of color saturation of a box is positively correlated with the enrichment level of the term. Solid, dashed, and dotted lines represent two, one, and zero enriched terms at both ends connected by the line, respectively. The rank direction of the graph is set from top to bottom. Figure S4 Hierarchical tree graph of over-represented GO terms in the biological process category of up-regulated DEGS. Boxes in the graph represent GO terms labeled according to their GO ID, term definition, and statistical information. Significant terms (adjusted P ≤ 0.05) are in color (red, orange, or yellow), while non-significant terms are shown as white boxes. In the diagram, the degree of color saturation of a box is positively correlated with the enrichment level of the term. Solid, dashed, and dotted lines represent two, one, and zero enriched terms at both ends connected by the line, respectively. The rank direction of the graph is set from top to bottom.

附加文件3：图S1。组装得到的单基因簇的基因本体论（Gene Ontology, GO）分类。纵轴代表单基因簇的数量，横轴代表GO分类条目。
图S2 组装得到的单基因簇的KEGG（Kyoto Encyclopedia of Genes and Genomes）通路功能分类。该KEGG通路被划分为6个功能大类：A- 新陈代谢；B- 遗传信息处理；C- 环境信息处理；D- 细胞过程；E- 生物系统；F- 人类疾病。纵轴代表KEGG代谢通路，横轴代表注释到对应通路的单基因簇数量。
图S3 下调差异表达基因（Differentially Expressed Genes, DEGs）的生物过程类别中富集的GO术语的层级树状图。图中的方框代表GO术语，标注内容包含其GO编号、术语定义以及统计信息。校正P值≤0.05的显著术语以彩色（红、橙或黄）标注，非显著术语则以白色方框展示。图中，方框的颜色饱和度与该术语的富集程度呈正相关。实线、虚线和点虚线分别代表连线两端各存在2个、1个和0个富集术语。该图的排序方向为从上至下。
图S4 上调差异表达基因的生物过程类别中富集的GO术语的层级树状图。图中的方框代表GO术语，标注内容包含其GO编号、术语定义以及统计信息。校正P值≤0.05的显著术语以彩色（红、橙或黄）标注，非显著术语则以白色方框展示。图中，方框的颜色饱和度与该术语的富集程度呈正相关。实线、虚线和点虚线分别代表连线两端各存在2个、1个和0个富集术语。该图的排序方向为从上至下。