Comparing genome-wide H3K36me3 levels in WT vs. dKDM4A mutant

dKDM4A is a Drosophila H3 K9/K36 me2/me3 demethylase, which has been disrupted here by P-element insertion. We compare whole-genome ChIP-chip in mutant and WT to look for target genes of dKDM4A. Two samples, WT and dkDM4A mutant. 2 biological replicates each. 2 arrays.

dKDM4A是果蝇（Drosophila）的H3 K9/K36二甲基化/三甲基化去甲基化酶，本研究通过P因子（P-element）插入使其功能失活。我们对突变体与野生型（WT，Wild Type）样本开展全基因组ChIP-chip（染色质免疫沉淀芯片，Chromatin Immunoprecipitation on chip）分析并进行对比，以鉴定dKDM4A的靶基因。本次实验共设置两组样本：野生型与dKDM4A突变体，每组均包含2次生物学重复，共计使用2张芯片。