An OTX2-PAX3 signaling axis regulates Group 3 medulloblastoma cell fate [scRNA-seq]

scRNA-seq was performed to evaluate the OTX2-PAX3-mTOR signalling axis at the single cell level in heterogeneous Group 3 MB tumorspheres Overall design: Tumorspheres were generated from 3 Group 3 MB cell lines to examine mTOR signalling and assess similiarity to patient tumors

本研究通过单细胞RNA测序（single-cell RNA sequencing）技术，在异质性第3组髓母细胞瘤（Group 3 MB）肿瘤球的单细胞水平，对OTX2-PAX3-哺乳动物雷帕霉素靶蛋白（mTOR）信号轴进行评估。
实验设计：本研究从3株第3组髓母细胞瘤细胞系中培养生成肿瘤球，以检测mTOR信号通路活性，并评估其与患者肿瘤的相似性。