RNA-Seq in Jurkat treated with either PBIT or DMSO

To assess whether genes associated with BH4Ds are more or less sensitive than genes associated with sharp H3K4me3 peaks to perturbation of H3K4me3 levels, we performed RNA-Seq experiment from Jurkat cells treated with DMSO or with 2-(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT), a specific inhibitor of JARID1 family of H3K4me3 demethylases, also known as KDM5 (Blair et al. 2011; Sayegh et al. 2013). Overall design: 3 samples in singlicate.

为探究与BH4Ds相关的基因相较于与尖锐H3K4me3峰相关的基因，对H3K4me3水平扰动的敏感性差异，我们对经二甲基亚砜（DMSO）或2-(4-甲基苯基)-1,2-苯并异噻唑啉-3(2H)-酮（PBIT）处理的Jurkat细胞开展了RNA测序（RNA-Seq）实验。其中PBIT是H3K4me3去甲基化酶JARID1家族（又称KDM5家族，Blair等，2011；Sayegh等，2013）的特异性抑制剂。实验总体设计：共设置3份单重复样本。