ATAC seq data. ATAC seq data

Multiple myeloma is associated with an immunosuppressive bone marrow microenvironment instigated and maintained by crosstalk between multiple myeloma cells and the surrounding cells. This crosstalk is facilitated or hindered by epigenetic modifiers altering the gene expression of receptors. The Polycomb-like protein PHF19/PCL3, which mediates polycomb repressive complex 2 (PRC2) recruitment to chromatin, is a high-risk gene whose expression level correlates with poor prognosis in MM patients. Here, we used ATAC seq to define the chromatin accessibility and transcriptional landscape controlling this process by PHF19. Overall design: ATAC-seq profiles of multiple myeloma cells with PHF19 overexpression and empty vectors types.

多发性骨髓瘤（Multiple myeloma, MM）与免疫抑制性骨髓微环境密切相关，该微环境由多发性骨髓瘤细胞与周围细胞之间的细胞串扰引发并维持。表观遗传调控因子通过改变受体基因的表达，对该细胞串扰起到促进或抑制作用。介导多梳抑制复合体2（Polycomb Repressive Complex 2, PRC2）招募至染色质的多梳样蛋白PHF19/PCL3属于高危基因，其表达水平与MM患者的不良预后相关。本研究采用ATAC-seq（转座酶可及性染色质测序，Assay for Transposase-Accessible Chromatin using sequencing）技术，解析了PHF19调控该过程的染色质可及性特征与转录调控全景。实验整体设计：对过表达PHF19的多发性骨髓瘤细胞与转染空载体的对照组细胞进行ATAC-seq测序，获取其对应的染色质开放图谱。