Additional file 1 of Loss of Tdp-43 disrupts the axonal transcriptome of motoneurons accompanied by impaired axonal translation and mitochondria function

Additional file 1: Supplementary Table 1. List of primers for cloning of shRNAs into pSIH. siRNA sequences are underlined. Supplementary Table 2. List of qPCR primers. Supplementary Table 3. List of transcripts significantly (p<0.05) upregulated in the somatodendritic compartment of Tdp-43 knockdown (T_sd) motoneurons relative to control (G_sd) motoneurons. Supplementary Table 4. List of transcripts significantly (p<0.05) downregulated in the somatodendritic compartment of Tdp-43 knockdown (T_sd) motoneurons relative to control (G_sd) motoneurons. Supplementary Table 5. List of transcripts significantly (p<0.05) upregulated in the axonal compartment of Tdp-43 knockdown (T_ax) motoneurons relative to control (G_ax) motoneurons. Supplementary Table 6. List of transcripts significantly (p<0.05) downregulated in the axonal compartment of Tdp-43 knockdown (T_ax) motoneurons relative to control (G_ax) motoneurons. Supplementary Table 7. List of transcripts significantly (p<0.05) deregulated in the somatodendritic (sd) and axonal (ax) compartment of Tdp-43 knockdown motoneurons relative to control motoneurons.

附加文件1：补充表1。用于将短发卡RNA（short hairpin RNA, shRNA）克隆至pSIH载体的引物列表，小干扰RNA（small interfering RNA, siRNA）序列已加下划线标注。补充表2：实时定量聚合酶链反应（quantitative real-time polymerase chain reaction, qPCR）引物列表。补充表3：相较于对照（G_sd）运动神经元，TAR DNA结合蛋白43（Tdp-43）基因敲低（T_sd）运动神经元的树突胞体区中显著（p<0.05）上调的转录本列表。补充表4：相较于对照（G_sd）运动神经元，Tdp-43基因敲低（T_sd）运动神经元的树突胞体区中显著（p<0.05）下调的转录本列表。补充表5：相较于对照（G_ax）运动神经元，Tdp-43基因敲低（T_ax）运动神经元的轴突区中显著（p<0.05）上调的转录本列表。补充表6：相较于对照（G_ax）运动神经元，Tdp-43基因敲低（T_ax）运动神经元的轴突区中显著（p<0.05）下调的转录本列表。补充表7：相较于对照运动神经元，Tdp-43基因敲低运动神经元的树突胞体（sd）与轴突（ax）区中显著（p<0.05）异常调控的转录本列表。