Werner Helicase Prevents Cell Death in Cancers with Microsatellite Instability [PacBio]

Our study uncovers a distinct biomarker for MSI that underlies the synthetic lethal dependence on WRN, thereby supporting the development of WRN-based therapeutics Pacbio CLR was performed under different experimental condition in MSI and MSS cells to uncover expansions/deletions at TA dinucleontide repeats genome-wide

本研究发现了一种针对微卫星不稳定性（Microsatellite Instability, MSI）的独特生物标志物，该标志物是细胞对沃纳综合征ATP依赖解旋酶（Werner syndrome ATP-dependent helicase, WRN）产生合成致死依赖的分子基础，从而为基于WRN的治疗药物开发提供了理论支撑。同时，本研究在MSI与微卫星稳定（Microsatellite Stable, MSS）细胞中设置不同实验条件开展了PacBio连续长读长（Continuous Long Read, CLR）测序，以在全基因组范围内检测TA二核苷酸重复序列的扩增与缺失事件。