MinKNOW + Porechop nanopore reads
收藏资源简介:
These files contain the nanopore long reads produced by R9.4 MinION, basecalled during sequencing by MinKNOW (v.Jun17) and demultiplexed by Porechop v0.2.1.<br>Half of each strain's sample underwent end repair, and half did not - these were sequenced under different barcodes. The samples here have had the reads from both barcodes pooled (see "Albacore + Porechop nanopore reads" for the individual readsets).<br><br>These reads were superseded by those from the more accurate basecaller, Albacore (see "Albacore only nanopore reads" for like-for-like equivalents).
本批次文件包含由R9.4型MinION测序仪生成的纳米孔长读长序列(nanopore long reads),所有序列在测序阶段通过MinKNOW(v.Jun17版本)完成碱基识别,并由Porechop v0.2.1完成解复用。 每个菌株的样本均分为两组:一半进行了末端修复处理,另一半未进行该处理,两组分别使用不同的条形码标记后完成测序。本次提供的样本已将两种条形码对应的序列数据合并,如需获取单独的序列数据集,请参考"Albacore + Porechop 纳米孔测序序列"。 本批序列已被准确率更高的碱基识别工具Albacore生成的序列所替代,如需获取同条件的等效数据集,请参考"仅Albacore处理的纳米孔测序序列"。



