High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [GM12878]

While recent technical advancements have facilitated the mapping of epigenomes at the single-cell resolution, the throughput and quality of these methods have limited the widespread application of these technologies. Here, we describe a high-throughput platform for single-cell assay for transposase accessible chromatin (scATAC-seq) using droplet microfluidics that yields essential qualities for high-throughput profiling, including improvements in per-cell library complexity and throughput. This approach enables robust cell-typing of complex tissues and reveals a de novo atlas of cellular and epigenomic diversity across many cell types and cell stages. Overall design: Variable bead concentrations and loading conditions for testing species mixing and data quality from SureCell scATAC-seq on GM12878 cells

尽管近年来技术进步已推动了单细胞分辨率下表观基因组的图谱绘制，但现有方法的通量与质量仍限制了这些技术的大规模应用。
本文报道了一种基于液滴微流控（droplet microfluidics）的单细胞转座酶可及性染色质测序（scATAC-seq）高通量平台，该平台具备高通量谱分析所需的核心特性，包括提升了单细胞文库复杂度与测序通量。
此方法可实现复杂组织的稳定可靠细胞分型，并绘制出覆盖多种细胞类型与细胞阶段的全新细胞及表观基因组多样性图谱。
实验设计：通过调整磁珠浓度与上样条件，基于SureCell scATAC-seq平台对GM12878细胞开展物种混合实验与数据质量检测。