Mouse Laser-Induced CNV Assay.

(A) Subretinal injection of MSA-CEP does not increase CNV area compared to mice injected with saline or MSA-CTL2. Bar graph shows mean area of CNV +/− SEM from first experiment evaluating the effect of subretinal injection of saline, 0.5 µg of rhVEGF165, 3.8 µg of MSA-CTL2, 3.8 µg of MSA-CEP, or 6.6 µg of 4G3 (an anti-mVEGF antibody) on laser-induced CNV in C57BL/6J mice. The number above each bar is the percentage inhibition relative to average CNV area in mice injected with MSA-CTL2. Subretinal injection of VEGF increases CNV area and subretinal injection of an anti-mVEGF antibody inhibits CNV area. * p<0.05, **** p<0.0001 by ANOVA with a Dunnett’s post hoc analysis. (B) Representative fluorescent images of CNV lesions 7 days after laser from mice injected in the subretinal space with MSA-CEP, MSA-CTL2, VEGF or a VEGF Antibody as described above. Scale bar = 100 microns. CTL2, MSA-CTL2; CEP, MSA-CEP.

(A) 相较于注射生理盐水或MSA-CTL2的小鼠，视网膜下注射MSA-CEP并未增加脉络膜新生血管（CNV）面积。本柱状图呈现了首个实验中各组小鼠的CNV平均面积±标准误（SEM），该实验评估了视网膜下注射生理盐水、0.5 μg重组人血管内皮生长因子165（rhVEGF165）、3.8 μg MSA-CTL2、3.8 μg MSA-CEP或6.6 μg 4G3（一种抗小鼠VEGF抗体）对C57BL/6J小鼠激光诱导脉络膜新生血管（laser-induced CNV）的影响。每个柱状图上方的数值为相对于MSA-CTL2注射组小鼠平均CNV面积的抑制百分比。视网膜下注射VEGF可扩大CNV面积，而视网膜下注射抗VEGF抗体则可抑制CNV面积。经方差分析（ANOVA）结合Dunnett事后检验，*p<0.05，****p<0.0001。
(B) 如上所述，对C57BL/6J小鼠视网膜下间隙注射MSA-CEP、MSA-CTL2、VEGF或抗VEGF抗体后，可得激光造模7天的CNV病灶代表性荧光图像。比例尺=100微米。注：CTL2指代MSA-CTL2；CEP指代MSA-CEP。