Transcriptional correlates of FOXP3 expression in suppressive and non-suppressive primary human Treg cell clones.

Analysis of the transcriptional correlates of FOXP3 expression in suppressive and non-suppressive primary human Treg cell clones. Individual CD4+CD25High or Cd4+CD25- T cells were isolated from human PBMCs and expanded in vitro. After 3 weeks of expansion, individual clones were analysed for FOXP3 expression and in vitro suppressive activity against freshly sorted allogeneic effector T cells. This study analyses the total RNA isolated from FOXP3+ clones with suppressive potency to their non-suppressive counterparts. The resutls of this study should provide insights into the molecular pathways linking FOXP3 expression to distinct aspects of Treg phenotype and function. Total RNA obtained from individual clones of primary human regulatory and effector CD4+T cells.

本研究针对人原代调节性T细胞（Treg）克隆中FOXP3表达的转录相关性展开分析，对象涵盖具有抑制功能与非抑制功能的两类克隆。实验从人外周血单个核细胞（PBMC）中分离单个CD4+CD25High或CD4+CD25- T细胞并进行体外扩增；扩增3周后，对各单个克隆分别检测其FOXP3表达水平，以及针对新鲜分选的同种异体效应T细胞的体外抑制活性。本研究对具有抑制活性的FOXP3+克隆与其非抑制性对应克隆中提取的总RNA开展分析，所得结果有望揭示将FOXP3表达与调节性T细胞表型及功能的不同维度相关联的分子通路。本数据集包含从原代人调节性与效应性CD4+T细胞单个克隆中提取的总RNA。