BAP1/ASXL complex modulation regulates Epithelial-Mesenchymal Transition during trophoblast differentiation and invasion. BAP1/ASXL complex modulation regulates Epithelial-Mesenchymal Transition during trophoblast differentiation and invasion

The experiment shows the transcriptional changes between mouse trophoblast stem cells transduced with non-targeting single-guide RNA (NT-sgRNA) or sgRNA against the promoter of the Bap1 gene (sgRNA1) at two different time points, 0 days (0d) -stem cell conditions- and after 3 days (3d) of differentiation Overall design: RNA-seq experiment in triplicate comparing control (NT-sgRNA) with Bap1- overexpressing mTSCs (sgRNA1) at two different time points, 0 days and 3 days of differentiation

本实验揭示了转导非靶向单引导RNA（non-targeting single-guide RNA, NT-sgRNA）或靶向Bap1基因启动子的单引导RNA（sgRNA1）的小鼠滋养层干细胞（mouse trophoblast stem cells）在两种时间节点的转录组变化：0天（干细胞维持培养条件，即未分化状态）与诱导分化3天（3d）后。
实验整体设计：本实验通过设置三次生物学重复的RNA测序（RNA-seq），在0天和诱导分化3天两个时间点，对比对照组（转导NT-sgRNA）与转导sgRNA1的Bap1过表达小鼠滋养层干细胞（mTSCs）的转录组差异。