Transcriptome dynamics of protein-coding genes during flower development in Arabidopsis. Transcriptome dynamics of protein-coding genes during flower development in Arabidopsis

Flower development is a dynamics process in which floral organs are produced from pools of stem cells residing in meristems (Smyth et al., 1990). In order to obtain a high resolution map of the changes of gene expression during this process thus to provide insights into specific expression patterns and their underlying gene regulatory networks, an inducible system which allows us to obtain synchronized flowers (Wellmer et al., 2006) was used to collect stage-specific floral tissues at four stages (stages 0, 2, 4 and 8) for transcriptome profiling by RNA-seq . These stages represent the status of inflorescence meristem, floral meristem specification, floral organ specification and floral organ differentiation, respectively during Arabidopsis flower development. Overall design: We used the AP1-GR system to conduct RNA-seq experiments at different stages of flower development. Samples were generated from tissue in which the AP1-GR protein was induced using a treatment of 1 uM DEX to the shoot apex. The material was collect before treatment and 2, 4 and 8 days after treatment. Experiments were done in two or three biological replicates.

花发育是一个动态过程，由定位于分生组织的干细胞池分化产生花器官（Smyth等，1990）。为获取该过程中基因表达变化的高分辨率图谱，进而阐明特异表达模式及其下游基因调控网络，本研究采用可诱导花同步化体系（Wellmer等，2006），收集了4个发育阶段（0、2、4和8期）的阶段特异性花组织，用于RNA测序（RNA-seq）转录组分析。上述四个阶段分别对应拟南芥花发育过程中的花序分生组织状态、花分生组织特化、花器官特化及花器官分化阶段。实验设计概述：本研究借助AP1-GR体系开展拟南芥花发育不同阶段的RNA-seq实验。实验样品取自经1μM地塞米松（DEX）处理茎尖以诱导AP1-GR蛋白表达的组织，分别于处理前及处理后2、4、8天采集样本。所有实验均设置2至3次生物学重复。