male_mid_L4_25dC_36hrs_post-L1_CB4689_tiling_array

modENCODE_submission_478 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: dpy28(y1);him-8(e1489); Developmental Stage: Male larva mid-L4 25dC 30 hrs post-L1; Genotype: dpy-28(y1);him-8(e1489); Sex: Male; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain dpy28(y1);him-8(e1489); Developmental Stage Male larva mid-L4 25dC 30 hrs post-L1

modENCODE提交项478 本提交项源自Robert Waterston的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本实验通过将RNA与商用基因组平铺芯片进行杂交，旨在检测秀丽隐杆线虫（C. elegans）的全部转录本。为最大化检测特定细胞中低表达稀有转录本的成功率，本研究通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）分离特定胚胎细胞，并采用信使RNA（mRNA）标记法分离胚胎后细胞，进而从中提取RNA。
有关数据使用条款与细则，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
关键词：转录本平铺芯片分析（Transcript tiling array analysis）
实验类型：转录本平铺芯片分析。
生物来源：品系：dpy28(y1);him-8(e1489); 发育阶段：雄性幼虫L4中期，25℃条件下L1期后培养30小时；基因型：dpy-28(y1);him-8(e1489); 性别：雄性；
生物学重复次数：3次；
实验因素：品系dpy28(y1);him-8(e1489); 发育阶段为雄性幼虫L4中期，25℃条件下L1期后培养30小时。