Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet

Purpose: Observing the response of the heart to dietary interventation at the metabolomic and transcriptomic level may have relevance for cardiovascular diseases Methods: RNA Seq of 12 week old WT male mice on chow or HFD and WT female mice on Chow or HFD; RNA was extracted and reverse-transcribed after polyA selection to complementary DNA for sequencing on an Illumina NovaSeq platform Results: RNAseq from heart tissue revealed significant differences in expression of 292 mRNA, with an FDR <0.05. PCA showed that there were significant sex differences in chow-fed mice. It also separated HFD-fed vs. Chow-fed samples for both male and female mice. Conclusions: This work reveals the response of a vital organ to dietary intervention at both metabolomic and transcriptomic levels, which is a fundamental question in physiology that may have relevance for cardiovascular diseases. This work also reveals significant sex differences in cardiac metabolites and gene expression. Overall design: RNA Seq of 12 week old WT male mice on Chow or HFD and WT female mice on Chow or HFD

研究目的：探究心脏在膳食干预下的应答反应，从代谢组学（metabolomic）与转录组学（transcriptomic）层面开展分析，其研究结果或可为心血管疾病相关研究提供参考。
实验方法：对12周龄的野生型（Wild Type, WT）雄性小鼠分别喂食普通维持饲料（Chow）与高脂饲料（High-Fat Diet, HFD），同时对同周龄的野生型雌性小鼠进行相同分组喂食；提取各组小鼠的RNA，经polyA富集后反转录为互补DNA（cDNA），随后使用Illumina NovaSeq测序平台进行RNA测序（RNA Seq）。
实验结果：对心脏组织的RNA测序分析显示，共有292个mRNA的表达量存在显著差异，错误发现率（False Discovery Rate, FDR）<0.05。主成分分析（Principal Component Analysis, PCA）结果表明，喂食普通饲料的小鼠中存在显著的性别差异；同时，无论雄性还是雌性小鼠，喂食高脂饲料与普通饲料的样本均可被明显区分。
研究结论：本研究揭示了重要脏器心脏在膳食干预下的代谢组学与转录组学层面的应答反应，这一生理学基础问题的研究结果或对心血管疾病相关领域具有参考价值。同时，本研究还发现心脏代谢物与基因表达存在显著的性别差异。
实验整体设计：对12周龄的野生型雄性小鼠分别喂食普通维持饲料或高脂饲料，同时对同周龄野生型雌性小鼠进行相同分组喂食。