ChIP-DSL H20K promoter array from human gastric cancer cells MKN28 with Sox2 overexpression. Homo sapiens

The transcription factor Sox2 inhibits human gastric cancer growth and activates Sox2-related tumor surpressive genes in human gastric cancer cells. Conditional Sox2-overexpression in cells with a low Sox2 level demonstrated that the Sox2-regulated tumor surpressive genes demand on an enhanced Sox2 activity for better expression to work in human gastric cancer. Chromatin immunoprecipitation (ChIP) of Sox2 together with chromatin profiling by ChIP-on-chip analysis demonstrated that Sox2 directly activates the chromatin at promoters or putative enhancers of Sox2 target genes. Overall design: Transcription factor Sox2 promoter array in MKN28 cells with Sox2 overexpression.转录因子Sox2（transcription factor Sox2）可抑制人胃癌细胞的生长，并激活人胃癌细胞内与Sox2相关的肿瘤抑制基因。针对Sox2低表达细胞开展的Sox2条件性过表达实验证实：Sox2调控的肿瘤抑制基因依赖于更高水平的Sox2活性，才能实现更优的表达并在人胃癌中发挥功能。通过对Sox2进行染色质免疫共沉淀（chromatin immunoprecipitation，ChIP）联合ChIP-on-chip染色质谱分析，结果显示Sox2可直接激活其靶基因启动子区域或潜在增强子区域的染色质。整体实验设计：在MKN28细胞中过表达Sox2，采用转录因子Sox2启动子阵列开展相关检测。