Data_Sheet_1_Proteomic Changes in Antioxidant System in Strawberry During Ripening.xlsx

To investigate the strawberry antioxidant defense system during fruit ripening, a targeted quantitative proteomic approach using multiple reaction monitoring (MRM) was developed to investigate targeted proteins in the antioxidant enzyme system in strawberry fruit. We investigated 46 proteins and isoforms with 73 identified peptides which may be involved in this antioxidant enzyme system. Among the proteins that changed during ripening, aldo/keto reductase (AKR), superoxide dismutase (SOD) and glutathione transferase (GT) increased significantly, while dehydroascorbate reductase, 2-Cys peroxiredoxin, catalase (CAT), 1-Cys peroxiredoxin and L-ascorbate peroxidase (APX) decreased significantly. These results suggest that fruit ripening of strawberry activates the enzymes of an SOD/glutathione metabolism system. The methodologies used in this study will be useful for systematically characterizing the role of antioxidant enzymes in fruit ripening of other plants.

为探究草莓果实成熟过程中的抗氧化防御系统，本研究开发了一种采用多反应监测（multiple reaction monitoring, MRM）技术的靶向定量蛋白质组学方法，用于分析草莓果实抗氧化酶系统中的靶向蛋白。本研究共分析了46种可能参与该抗氧化酶系统的蛋白质及其同工型，共鉴定得到73条肽段。在果实成熟过程中表达量发生变化的蛋白质中，醛酮还原酶（aldo/keto reductase, AKR）、超氧化物歧化酶（superoxide dismutase, SOD）以及谷胱甘肽转移酶（glutathione transferase, GT）的表达量显著上调；而脱氢抗坏血酸还原酶、2-半胱氨酸过氧化物还原酶、过氧化氢酶（catalase, CAT）、1-半胱氨酸过氧化物还原酶以及L-抗坏血酸过氧化物酶（L-ascorbate peroxidase, APX）的表达量则显著下调。上述结果表明，草莓果实成熟过程可激活超氧化物歧化酶/谷胱甘肽代谢系统中的酶活性。本研究采用的方法学将有助于系统解析其他植物果实成熟过程中抗氧化酶的功能作用。