Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Kidney with CODEX, v1.0

The OMAP kidney panel was designed for CODEX (CO Detection by IndEXing) of fresh frozen human kidney samples. The<br>panel contains 24 antibodies and the nuclear marker Hoechst 33342 or DAPI for image alignment and nuclear<br>segmentation. This OMAP provides a spatial context for all anatomical structures and most cell types present in the<br>[ASCT+B kidney table v1.1](https://doi.org/10.48539/HBM673.ZZDK.622). Additionally, the inclusion of protein<br>biomarkers tryptase and MARCKS allow profiling of different regions in glomeruli that are often affected by disease<br>as well as several immune cell markers that are indicative of health. The core and essential protein biomarkers<br>detailed here overlap with panels developed for multiplexed imaging of kidney samples using other technologies, such<br>as cyTOF and cyclic multiplexed immunofluorescence.

本OMAP肾脏抗体组合专为新鲜冷冻人肾脏样本的CODEX（CO Detection by IndEXing，即通过索引实现共检测）成像检测而设计。
该组合包含24种特异性抗体，以及用于图像配准与细胞核分割的核标记物Hoechst 33342或DAPI。
本组合可为[ASCT+B肾脏表v1.1（https://doi.org/10.48539/HBM673.ZZDK.622）]中收录的所有解剖结构及多数细胞类型提供空间分布背景信息。
此外，该组合纳入了蛋白生物标记物类胰蛋白酶（tryptase）与MARCKS，可用于分析常受疾病累及的肾小球不同区域；同时还包含数种可反映机体健康状态的免疫细胞标记物。
本数据集详述的核心必需蛋白生物标记物，与采用其他技术（如飞行时间流式细胞术（cyTOF）及循环多重免疫荧光技术）开展肾脏样本多重成像研究所用的抗体组合存在重叠。