Comparative proteomics analysis of decidua tissues in early recurrent pregnancy loss

The project aimed to determine proteomics changes in decidua from patients suffering from unexplained early recurrent pregnancy loss (RPL) compared to women undergoing elective abortions. Comparative proteomics analysis by label-free data-independent LC-MS/MS was performed on fresh frozen decidua tissues from 19 RPL patients and 10 controls. Samples from EPL patients were preselected to include only patients with 2 or more recurrent pregnancy losses (RPL), no previous live birth, gestational week from 6-10 weeks and excluded fetal chromosomal abnormalities. Control samples were from women without history of previous miscarriage, ectopic pregnancy or preterm delivery, with at least one live born child, at 6-10 gestational weeks and without fetal chromosomal abnormalities. Patients and controls had no significant differences regarding age (Median age RPL = 30; Median age Controls = 34; Mann–Whitney U-test p = 0.082). The mean gestational weak (GW) was 7.9±1.0 weeks in the RPL group and 8.3±1.2 weeks in the control group, without statistically significant difference between groups (Median GW RPL = 8; Median GW Controls = 8; Mann–Whitney U-test p = 0.361).

本研究旨在探究不明原因早期复发性流产（unexplained early recurrent pregnancy loss, RPL）患者蜕膜组织的蛋白质组学变化，并与接受择期流产的女性进行对照分析。研究采用无标记数据非依赖型液相色谱-串联质谱（label-free data-independent LC-MS/MS）技术，对19例RPL患者及10例对照者的新鲜冰冻蜕膜组织开展比较蛋白质组学研究。
受试的RPL患者样本经预先筛选，仅纳入复发性流产次数≥2次、无既往活产史、孕周处于6~10周且排除胎儿染色体异常的受试者。对照组样本来源于无流产史、异位妊娠史或早产史，育有至少1名活产子女，孕周为6~10周且无胎儿染色体异常的女性。
患者与对照者的年龄无显著统计学差异：RPL组年龄中位数为30岁，对照组为34岁（曼-惠特尼U检验，Mann–Whitney U-test，p=0.082）。RPL组平均孕周为7.9±1.0周，对照组为8.3±1.2周；两组孕周中位数均为8周，组间差异无统计学意义（曼-惠特尼U检验，Mann–Whitney U-test，p=0.361）。