Triple-combination therapy for cutaneous leishmaniasis using detergent-free, hyaluronate-coated elastic nanovesicles - Supplementary data set

Aim: To develop and evaluate detergent-free, triple-drug-loaded, hyaluronate-coated elastic nanovesicles (H-ENVs) for the topical treatment of cutaneous leishmaniasis. Materials & methods: H-ENVs were developed and evaluated for vesicle size, entrapment efficiency, skin permeation and antileishmanial potential. Results: A 15.7 and 28.6% decrease in the cytotoxicity of paromomycin and amphotericin B, respectively, was observed in detergent-free ENVs compared with conventional ENVs. H-ENVs improved the efficacy of paromomycin against promastigote and amastigote models of leishmaniasis by 4- and 7.5-fold, respectively. In vivo investigation of H-ENVs demonstrated efficient topical management of cutaneous leishmaniasis. Conclusion: The results indicate the potential of H-ENVs as a safe topical treatment choice for cutaneous leishmaniasis.研究目的：开发并评估无洗涤剂、负载三重药物的透明质酸包被弹性纳米囊泡（hyaluronate-coated elastic nanovesicles，H-ENVs），用于皮肤利什曼病的局部治疗。 材料与方法：制备H-ENVs，并对其囊泡粒径、包封率、皮肤渗透性能及抗利什曼原虫活性进行评价。 研究结果：相较于传统弹性纳米囊泡，无洗涤剂弹性纳米囊泡中的巴龙霉素与两性霉素B的细胞毒性分别降低15.7%与28.6%。H-ENVs可使巴龙霉素对利什曼原虫前鞭毛体和无鞭毛体模型的抗虫功效分别提升4倍和7.5倍。体内实验证实，H-ENVs可有效实现皮肤利什曼病的局部管理。 结论：本研究结果表明，H-ENVs具备作为皮肤利什曼病安全局部治疗方案的潜力。