Proteomic profile of cytosolic and endocytic protein distribution in sorted populations of reticulocytes and extruded nuclei.

Sorted populations of reticulocytes and extruded nuclei were fractionated by 1D SDS-PAGE and subjected to Nano LC mass spectrometry. An abridged list containing key cytosolic and endocytic proteins of interest is shown. Total peptide column is the total number of peptides (and therefore an indication of a particular protein’s abundance) detected in the population, whilst the unique peptide column indicates the number of unique peptides detected. To assess differences between nuclei and reticulocyte populations the total peptide number should be used.经分选的网织红细胞（reticulocytes）与脱核排出的细胞核群体，通过1D SDS-PAGE进行分级分离，并经纳升液相色谱-质谱联用（Nano LC mass spectrometry）分析。本文展示了一份包含所关注关键胞质蛋白与内吞蛋白的精简列表。总肽段列代表该群体中检测到的肽段总数（可作为特定蛋白丰度的指示指标），而唯一肽段列则代表检测到的唯一肽段数量。若需评估细胞核与网织红细胞群体间的差异，应采用总肽段数。