SH1410093.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定物种、识别类群、交流信息以及处理与基于DNA的物种假说（DNA-based Species Hypotheses，以下简称SH）相关的各类工作。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一套动态距离阈值（0.5%~3.0%）聚类至近似物种级别的类群。所有物种假说均以数字对象标识符（Digital Object Identifier，DOI）的形式赋予唯一且稳定的名称，其分类学与生态学注释信息则通过分布式、基于网页的第三方注释工作完成验证。通过整合该SH包含的全部序列的鉴定结果，尽可能将SH与对应的分类单元名称及其门、纲、目等分类阶元进行关联。为每个SH选定一条自动或人工指定的代表序列。上述代表序列已通过链接https://unite.ut.ee/repository.php公开发布，可供科学界用于本地序列相似性搜索、高通量测序分析流程等研究场景。随着公开真菌ITS序列数量的持续增长，该系统与数据集将自动完成更新。