Main genetic variability measures by locus of Carcinus maenas from the Portuguese coast.

T (°C): annealing temperature; bp: base pairs; Na: number of alleles found per locus; HE: expected heterozygosity; HO: observed heterozygosity; FIS: standardised genetic variance within populations at each locus; FST: standardized genetic variance among populations at each locus; H-W: Hardy-Weinberg P values.*Microsatellites developed in the present study.**Minor adjustments to Tepolt et al. [30] F:AGGCGTCCTTTGTTTCAGTT; R:TTGATTTGATGTTACTTTTAGGATGT.T (℃)：退火温度（annealing temperature）；bp：碱基对（base pairs）；Na：每一位点检出的等位基因数（number of alleles found per locus）；HE：期望杂合度（expected heterozygosity）；HO：观测杂合度（observed heterozygosity）；FIS：各群体内单一位点的标准化遗传方差（standardised genetic variance within populations at each locus）；FST：各群体间单一位点的标准化遗传方差（standardized genetic variance among populations at each locus）；H-W：哈迪-温伯格P值（Hardy-Weinberg P values）。*本研究开发的微卫星标记（microsatellites）。**对Tepolt等人[30]的研究进行了小幅调整。正向引物（F）：AGGCGTCCTTTGTTTCAGTT；反向引物（R）：TTGATTTGATGTTACTTTTAGGATGT。