Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora

Chronic obstructive pulmonary disease (COPD) is a common obstructive respiratory disease characterized by persistent respiratory symptoms and limited airflow due to airway obstruction. The present study investigates the distribution characteristics of respiratory tract flora in both frequent and infrequent exacerbators of COPD. The 16S sequencing technique was adopted to differentiate the inherent differences of respiratory tract flora between frequent exacerbators and infrequent exacerbators. Additionally, cell counting kit 8 (CCK8), lactate dehydrogenase (LDH) test, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and western blot were carried out in human bronchial epithelial cells cultured in vitro and the regulatory effects of differential flora were verified.

慢性阻塞性肺疾病（Chronic obstructive pulmonary disease, COPD）是一类常见的阻塞性呼吸系统疾病，以持续呼吸道症状及气道阻塞所致的气流受限为典型特征。本研究聚焦慢性阻塞性肺疾病频繁急性加重者与非频繁急性加重者的呼吸道菌群分布特征，采用16S测序技术解析两组人群呼吸道菌群的固有差异。此外，本研究以体外培养的人支气管上皮细胞为实验模型，开展了细胞计数试剂盒8（CCK8）实验、乳酸脱氢酶（LDH）检测、流式细胞术、酶联免疫吸附试验（ELISA）及蛋白质印迹（Western blot）实验，验证了差异菌群的调控作用。