Functional In vivo Recording and Single-cell Transcriptome Analysis Reveals Molecular Properties of Light-Sensitive Neurons in Mouse V1

We established a protocol of functional in vivo single-cell transcriptome (FIST) analysis Overall design: Search for differential expressed genes defined the LS (light sensitive) and NS (non-light sensitive) neurons我们建立了功能体内单细胞转录组分析（FIST，functional in vivo single-cell transcriptome analysis）的实验方案。实验整体设计：通过筛选差异表达基因，界定光敏感（LS，light sensitive）与非光敏感（NS，non-light sensitive）神经元。