Absolute and relative values of autophagosomes per nuclei in human skin fibroblasts (CTRL + wIRA)

Our research hypothesis was that heat treatment by water-filtered infrared-A irradiation (wIRA) at 39 °C for 60 min would lead to increased autophagy compared to an untreated control. The following files show the treatment of human skin fibroblasts from a young healthy donor in three independent experiments to assess reproducibility. One file shows the absolute values of autophagy per nuclei in untreated and treated cells and the other file shows the relative values. All measurements were performed in six replicates, with at least three replicates used for data calculation. The data show a significant and reproducible increase in autophagosomes per nuclei by treatment with wIRA.本研究提出如下假设：相较于未处理的对照组，采用39℃、时长60分钟的水滤红外A辐照（water-filtered infrared-A，wIRA）热处理，可提升细胞自噬水平。以下文件展示了来自一名年轻健康供体的人皮肤成纤维细胞的处理过程，共开展三次独立重复实验以验证实验可重复性。其中一份文件记录了未处理组与处理组细胞中每个细胞核对应的细胞自噬绝对数值，另一份文件则记录了相对数值。所有测量均设置6次重复，其中至少3次重复用于数据计算。实验数据表明，经wIRA处理后，每个细胞核对应的自噬体数量出现了具有统计学意义且可重复的显著提升。