Type I conserved positions.

1Amino acid positions are numbered according to T. thermophilus EFG-2 structure 1WDT. An alternative numeration (EFG-1 of T. thermophilus) is given in brackets.
2Hydropathy index (positive value indicates hydrophobicity and negative value indicates hydrophilicity) [71].
3Amino acid conservation in is given in %.
*Substitutions of Ile with Val or Leu results in minimal change in hydrophobicity.
**Lys replacement by Arg retains positive charge in this position.

1. 氨基酸位点编号依照嗜热栖热菌（Thermus thermophilus）EFG-2的结构1WDT进行标注，备选编号方案（嗜热栖热菌的EFG-1）以括号形式给出。
2. 疏水指数（正值表示疏水性，负值表示亲水性）[71]。
3. 氨基酸保守性以百分比形式呈现。
* 异亮氨酸（Ile）被缬氨酸（Val）或亮氨酸（Leu）取代时，该位点的疏水性变化极小。
** 赖氨酸（Lys）被精氨酸（Arg）取代时，可保留该位点的正电荷。