Physiological transcriptional profiling of murine osteocytes from calvaria and long bones

Different bones of the skeleton originate from three distinct embryonic lineages. Osteoblasts derived from bones of different embryonic origin displayed cell intrinsic difference. Osteocytes are terminally differentiated osteoblasts which displays a unique genetic makeup and a distinct morphology. It is not know if osteocytes display a cell intrinsic differences depeding on their origin. To understand the difference in the gene expression profiles of osteocytes of three different embryonic origins we examined osteocytes from three different bones neural crest origin-frontal bone, paraxial mesoderm origin-parietal bone, and lateral plate mesoderm- femur bone. Overall design: Examine the changes in the transcriptome in frontal, parietal and femur osteocytes.

骨骼的不同骨组织源自三种截然不同的胚胎谱系。源自不同胚胎起源骨组织的成骨细胞（Osteoblasts）存在细胞内在差异。骨细胞（Osteocytes）是终末分化的成骨细胞，具有独特的基因表达特征与特异形态结构。目前尚不明确骨细胞是否会因其胚胎起源不同而表现出细胞内在差异。为探究三种不同胚胎起源骨细胞的基因表达谱差异，本研究选取了三类不同胚胎起源的骨组织来源的骨细胞：神经嵴（neural crest）起源的额骨（frontal bone）、轴旁中胚层（paraxial mesoderm）起源的顶骨（parietal bone），以及侧板中胚层（lateral plate mesoderm）起源的股骨（femur bone）。实验整体设计：分析额骨、顶骨与股骨骨细胞的转录组（transcriptome）变化。