Expression data from HELA cells subject to EGF stimulation. Homo sapiens

HELA cells derived from human cervical tumor were subjected to EGF stimulation for 0,20,40,60,120,240 and 480 minutes. We used microarrays to understand the temporal regulation of the cellular EGFR cascade. Keywords: time course Overall design: HELA cells were grown in DMEM supplemented with 10% FCS and antibiotics. Cells were serum deprived for 24 hours. Following stimulation with 10 ng/ml EGF for 0,20,40,60,120,240 and 480 minutes.将源自人宫颈肿瘤的海拉细胞（HeLa cells）分别以表皮生长因子（EGF）刺激0、20、40、60、120、240及480分钟。本研究利用微阵列（microarrays）技术解析细胞内EGFR信号级联的时序调控机制。关键词：时间进程（time course） 总体设计：海拉细胞于添加10%胎牛血清（FCS）及抗生素的达尔伯克改良伊格尔培养基（DMEM）中培养，经24小时血清饥饿处理后，以10 ng/ml浓度的EGF分别刺激0、20、40、60、120、240及480分钟。